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What is CD63 antibody?

Published in Antibody Characterization 2 mins read

CD63 antibody, specifically the CD63 Antibody (MX-49.129.5), is a mouse monoclonal IgG1 κ antibody that is used in research and diagnostic applications to detect the CD63 protein.

Key features of the CD63 antibody (MX-49.129.5):

  • Type: Mouse monoclonal IgG1 κ antibody
  • Concentration: 200 µg/ml
  • Source: Raised against full-length CD63 of human origin
  • Reactivity: Shown to detect CD63 in mouse, rat, and human samples.
  • Applications: Recommended for use in Western blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded sections) IHC(P), Flow Cytometry (FCM) and ELISA.
  • Usage: It has been cited in 572 publications, indicating its widespread use in research.

Applications Explained:

Here's a brief overview of some of the applications mentioned in the datasheet:

  • Western Blotting (WB): Used to identify and quantify the CD63 protein in a sample.
  • Immunoprecipitation (IP): Used to isolate the CD63 protein from a complex mixture.
  • Immunofluorescence (IF): Used to visualize the location of CD63 protein within cells.
  • Immunohistochemistry (IHC): Used to detect CD63 protein in tissue sections, often to study protein expression in diseased tissues like cancer.
  • Flow Cytometry (FCM): Used to identify and count cells expressing CD63.
  • ELISA: Used for quantitative detection of CD63.

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