Based on the provided video reference, using Megalign Pro involves specific actions for interacting with sequence alignments, such as viewing sequence gaps, navigating the display, and adding additional sequences.
While a comprehensive guide covers starting projects, running alignments, and analyzing results, the reference specifically highlights these operational uses:
Key Actions in Megalign Pro (According to Reference)
Megalign Pro allows users to manage and visualize sequence alignments effectively. The video snippet emphasizes the ability to inspect and expand existing projects.
Navigating and Viewing Alignments
A core function is the ability to closely examine the alignment data. This includes:
- Viewing Sequence Gaps: You can visualize and identify gaps present within the aligned sequences. Gaps often indicate insertions or deletions relative to other sequences in the alignment.
- Sliding the Viewport: The interface allows you to move or "slide" the viewing area (viewport) across the sequences. This is essential for navigating long alignments and visualizing different regions of the sequences that may not fit on the screen at once.
Adding Sequences to an Existing Alignment
A practical feature is the capability to update or expand ongoing projects:
- Aligning Additional Sequences: You have the power to align new sequences to an alignment that you have already created. This means you don't always need to start from scratch when incorporating new data; you can simply add it to your existing project.
These actions enable users to perform detailed examination of alignments and efficiently incorporate new data into their analyses within Megalign Pro.
Here is a summary of the uses mentioned in the reference:
| Action | Description | Purpose |
|---|---|---|
| Viewing Gaps | Display and identify gaps in aligned sequences | Helps locate potential insertions or deletions. |
| Sliding Viewport | Move the viewing area across the alignment | Allows visualization of different sequence regions. |
| Adding Sequences | Align new sequences to an existing project | Expands analysis without recreating the alignment. |
Understanding these functionalities is a fundamental step in utilizing Megalign Pro for sequence analysis.
