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How do you take an IV sample?

Published in Blood Sampling 4 mins read

Taking an IV sample, also known as venipuncture, involves drawing blood from a vein using a needle and syringe or vacutainer system. The process is crucial for diagnostic testing. While the reference provides some high-level steps on completing the process, such as filling out patient details on the vacutainer and sending off the sample, a more detailed step-by-step guide is helpful to understand the process. Below is a breakdown:

Steps to Take an IV Sample

  1. Preparation and Patient Identification:

    • Verify the patient's identity using multiple identifiers.
    • Explain the procedure to the patient, addressing any concerns.
    • Gather all necessary supplies: needles, syringes or vacutainer system, collection tubes, antiseptic wipes, gloves, gauze, tape, and sharps container.
    • Ensure you have filled out the patient details on the vacutainer bowl as noted in the video reference at [2:45] and [3:23].
  2. Hand Hygiene and Personal Protective Equipment (PPE):

    • Wash your hands thoroughly with soap and water or use an alcohol-based hand sanitizer.
    • Put on gloves.
  3. Vein Selection:

    • Apply a tourniquet 3-4 inches above the intended puncture site to make veins more prominent.
    • Palpate the area to locate a suitable vein. Choose a vein that is straight, palpable, and not rolling. The median cubital vein in the antecubital fossa (inner elbow area) is often a good choice.
    • Avoid areas with scars, tattoos, hematomas, or recent IV sites.
    • Clean the chosen site with an antiseptic wipe using a circular motion from the inside out and allow it to air dry completely. Do not touch the site again after cleaning.
  4. Venipuncture:

    • Hold the skin taut with your non-dominant hand just below the puncture site.
    • Insert the needle into the vein at a 15-30 degree angle with the bevel up.
    • Once you see a flash of blood in the syringe or vacutainer adapter, advance the needle slightly.
    • If using a syringe, gently pull back on the plunger to collect the desired amount of blood.
    • If using a vacutainer system, insert the appropriate collection tubes, allowing them to fill with blood. Note that the reference video mentions to fill out patient details on the vacutainer bowl.
  5. Post Venipuncture:

    • Remove the tourniquet once the required amount of blood is collected.
    • Remove the needle quickly while applying pressure on the puncture site with a clean gauze.
    • Activate the needle safety device to prevent needle stick injuries.
    • Discard the needle and syringe or vacutainer assembly into a sharps container.
    • Continue to apply pressure to the puncture site for 2-3 minutes to prevent hematoma formation.
    • Apply a bandage or tape to secure the gauze.
  6. Sample Handling and Lab Submission:

    • Gently invert blood tubes several times to mix the sample with the anticoagulant, if present.
    • Label each tube with the patient's name, date of birth, and other necessary information.
    • Ensure the blood sample is sent off to the lab for testing as mentioned in the reference at [3:23].
Step Description
Patient Identification Verify patient details.
Preparation Gather supplies and ensure proper hand hygiene and PPE.
Vein Selection Locate suitable vein, apply tourniquet, and clean the area.
Venipuncture Insert needle into vein and draw blood.
Post Venipuncture Remove needle, apply pressure, and bandage.
Sample Handling Mix blood samples, label tubes, and send for testing.

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