To grow NK cells, you need to culture them in a specific manner that promotes their proliferation. Here’s a breakdown of the process:
Culture Setup
The primary method involves using horizontally positioned flasks. Here's how to set up the culture:
- Cell Types: The culture requires two main cell types:
- A-NK cells: These are the natural killer cells that you want to propagate.
- Feeder cells: These cells provide necessary growth factors and support for the A-NK cells.
- Flask Placement: Position culture flasks horizontally without disturbance.
- Duration: Maintain the culture for 5-7 days.
Proliferation Process
During this incubation period, the following steps occur:
- Detachment: The A-NK cells detach from the plastic surface of the flask.
- Clump Formation: The detached A-NK cells start forming floating clumps with the feeder cells.
- Vigorous Proliferation: The cells then begin to multiply rapidly within these clumps.
Key Factors for Success
To ensure optimal NK cell growth:
- Feeder Cell Support: Feeder cells are crucial, as they release essential factors that stimulate NK cell growth.
- Undisturbed Culture: It’s important not to disturb the culture during the 5–7-day proliferation period.
- Observation: Regularly observe the floating cell clumps which indicate successful proliferation.
In summary, growing NK cells involves culturing them with feeder cells in horizontally positioned flasks for 5–7 days without disturbance, allowing them to detach, form clumps, and proliferate vigorously.
