The recommended density gradient medium, Ficoll®, has a density of 1.077 g/mL.
Density Gradient Mediums Explained
Density gradient mediums are crucial in various scientific applications for separating particles or cells based on their density. These mediums are solutions that form a gradient of increasing density from top to bottom. When a sample is layered on top of this gradient and centrifuged, the particles will move through the medium until they reach their respective density point, thus creating a separation.
Ficoll®: A Popular Choice
According to the reference, Ficoll® stands out as the most common density gradient medium used. This preference is attributed to its properties and effectiveness in cell separation processes.
- Density: Ficoll® provides a controlled density of 1.077 g/mL.
- Common Use: Its widespread adoption highlights its reliability and efficiency in cell separation.
- Reference: This is also highlighted in Homemade density gradient methods. Within the various density media available (Table 3), Ficoll® is the most commonly used, a medium with a density of 1.077 g/mL [69,118].
Benefits of Using Ficoll®
- Efficient Separation: Ficoll®'s specific gravity effectively separates cells and particles, resulting in high purity fractions.
- Versatility: It is used in a wide array of research and clinical applications.
- Biocompatibility: Generally, it is considered non-toxic to biological samples.
Other Density Gradient Media
While Ficoll® is commonly used, other media options are available. The choice of a density medium depends on the specific needs of the experiment or separation process. However, Ficoll's 1.077 g/mL density is frequently sought.
