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How is creatine kinase measured?

Published in Enzyme Measurement 2 mins read

Creatine kinase (CK) levels are measured through a blood test. Here's a detailed look at the process:

The Measurement Process

  1. Blood Sample Collection: A blood sample is drawn from the patient.
  2. Separation: The collected blood sample is then processed to separate its components into two fractions:
    • A fraction containing cells.
    • A fraction that does not contain cells, known as serum.
  3. CK Analysis: The serum is analyzed to determine the amount of creatine kinase present. The analysis measures enzyme activity.
  4. Reporting of Results: The amount of CK is reported in units (U) of enzyme activity per liter (L) of serum. So, the unit would look like U/L.


Step Description
1. Sample Collection Blood is drawn from the patient.
2. Fraction Separation Blood is separated into cell-containing and cell-free (serum) portions.
3. CK Analysis Serum is analyzed to measure the activity of creatine kinase.
4. Reporting CK levels reported as units per liter of serum (U/L).


Key Takeaways

  • Serum: The key to measuring CK is using the serum, the liquid part of the blood without cells.
  • Enzyme Activity: CK measurement focuses on the activity of the enzyme in breaking down creatine.
  • Units: The result is quantified using a standard measurement of enzyme activity per liter.

This method allows healthcare professionals to assess various conditions by observing any abnormal elevation in CK levels, which can indicate muscle damage, heart issues, or other health concerns.

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