The method for preserving fetal tissue depends on the intended use, but generally involves placing the specimen in a specific media or on sterile gauze. Here's a breakdown:
Immediate Preservation Methods
The following methods should be implemented immediately after tissue collection to ensure viability for downstream applications.
- Culture Media with Antibiotics: The preferred method is to place the fetal tissue specimen in culture media containing antibiotics. A 3X concentrated media is often recommended. This media helps maintain cell viability and prevents bacterial contamination. Contact the receiving laboratory to request this media if necessary.
- Moist Sterile Gauze: If culture media is unavailable, place the tissue on top of a sterile gauze pad that has been moistened with sterile water. Ensure the tissue is in a sterile container with a lid to prevent contamination and desiccation.
Important Considerations
- Avoid Submersion: DO NOT submerge the tissue in saline or water, as this can damage the cells.
- Sterility: Maintaining sterility throughout the process is crucial to prevent contamination and preserve the integrity of the tissue.
- Consultation: Always consult with the laboratory that will be receiving the tissue for their specific requirements and recommendations. They may have specific media or procedures they prefer.
