To collect a blood slide, follow these steps carefully to ensure a good smear for analysis:
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Prepare the Slide: Begin with a clean, labeled microscope slide. The slide should be pre-cleaned to avoid any artifacts. Label the slide near the frosted end.
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Apply the Blood Drop: Place a small drop of blood (about 2-3 mm in diameter) onto the slide, approximately 1/2 inch from the frosted end.
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Position the Spreader Slide: Hold a second clean slide (the "spreader slide") at a 30-45° angle to the first slide.
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Contact and Spread: Bring the spreader slide into contact with the first slide just in front of the blood drop. Allow the blood to spread along the line of contact between the two slides via capillary action.
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Create the Smear: Once the blood has spread almost to the edges of the spreader slide, quickly and smoothly push the spreader slide forward, away from the blood drop, towards the unfrosted end of the lower slide. The angle and speed of this movement affect the quality of the smear. A slower speed or higher angle results in a thicker smear.
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Air Dry: Allow the blood smear to air dry completely before staining or further analysis.
Key Considerations for a Good Blood Smear:
- Cleanliness: Ensure both slides are clean and free of grease or fingerprints.
- Blood Volume: Use the correct amount of blood. Too much or too little will result in a poor smear.
- Angle: Maintain a consistent angle between the slides.
- Speed: A smooth, even spreading motion is crucial. Avoid hesitation or stopping mid-smear.
- Drying: Allow the slide to air dry fully to prevent cellular distortion during staining.
