The solution used for Erythrocyte Sedimentation Rate (ESR) testing is Trisodium citrate.
Trisodium Citrate Solution for ESR
Trisodium citrate acts as an anticoagulant, preventing the blood from clotting, which is essential for accurately measuring the rate at which red blood cells settle in a tube over a specific period (usually one hour). The ESR test is a common hematology test that can help detect inflammation in the body.
Preparation of Trisodium Citrate Solution for ESR:
A common concentration is 3.8% Trisodium Citrate. This is often prepared as follows:
- Concentration: 3.8 grams of Trisodium citrate per 100 mL of water.
- Example: To prepare 500 mL of ESR solution, dissolve 19 grams of Trisodium citrate in 500 mL of distilled water.
Why Trisodium Citrate?
Trisodium citrate functions as an anticoagulant by binding calcium ions in the blood, preventing the coagulation cascade from occurring. This allows the red blood cells to settle freely, facilitating an accurate ESR measurement.
