No, you cannot use layers in Preview.
According to the reference, Preview doesn't offer a layering option. This means you can't combine multiple images into one file with individual, editable layers, similar to what you can do with image editing software like Photoshop or GIMP. While Preview is a versatile tool for viewing and making simple adjustments to images and PDFs, it is not designed for complex image manipulation involving layers.
However, the reference does offer a workaround to display two images side by side using Preview:
- Determine the total desired width of the combined images (e.g., 600 pixels if each image is 300 pixels wide).
- Open any image in Preview and adjust its width to the combined width (e.g. 600 pixels).
- Copy and paste each additional image to be shown next to each other.
This method does not use layers. Instead, it creates a single image where multiple images are positioned next to each other.