Serum iron is measured using a colorimetric reaction on a chemistry analyzer.
Detailed Measurement Process
The measurement of serum iron involves a specific chemical reaction that produces a colored compound. Here's a breakdown of the process:
- Colorimetric Reaction: Serum iron measurement relies on a colorimetric assay. This means that the amount of iron present is determined by the intensity of the color produced in a chemical reaction.
- Chromogen Usage: The key to this color development is the use of a chromogen. In this case, either ferrine or ferrozine is used as the chromogen.
- Complex Formation: When the chromogen (ferrine or ferrozine) reacts with the iron in the serum, it forms a colored complex.
- Quantification: The intensity of the color is directly proportional to the amount of iron present in the serum. This color intensity is measured by the chemistry analyzer, allowing for precise quantification of the serum iron concentration.
Simplified Explanation
Essentially, a chemical (ferrine or ferrozine) is added to the serum sample. This chemical reacts with any iron present, creating a colored solution. The intensity of the color indicates how much iron is present in the serum. This is measured using specialized equipment.
Why Colorimetry?
Colorimetry is used because it's a straightforward and reliable method for quantifying substances in liquid samples. It's widely employed in clinical labs due to its accuracy and efficiency.
| Step | Description |
|---|---|
| 1. Sample Preparation | Serum sample is obtained and prepared for analysis. |
| 2. Chromogen Addition | Ferrine or ferrozine chromogen is added to the serum sample. |
| 3. Color Complex Formation | The chromogen reacts with serum iron to form a colored complex. |
| 4. Measurement | The intensity of the color complex is measured using a chemistry analyzer. |
| 5. Quantification | The analyzer calculates the serum iron concentration based on color intensity. |
This method ensures accurate and repeatable measurement of serum iron, which is crucial for clinical diagnosis.
