No, Jane the Virgin does not lose her virginity before marriage.
Despite facing pressure and encouragement from multiple characters to explore her sexuality before marriage, Jane ultimately abstains from premarital sex throughout the series. According to the reference, "multiple characters encouraged Jane to lose her virginity before marriage, an option which she ultimately abstained from," highlighting her choice to remain a virgin until marriage. The series, however, emphasizes Jane's agency and control over her own sexuality, regardless of her decision to remain a virgin.
