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How to Do an Antigen Test?

Published in Medical Testing 4 mins read

An antigen test typically involves swabbing your nostrils to collect a sample and then processing it according to the test kit's instructions. Here's a step-by-step guide:

1. Preparation:

  • Read the Instructions: Carefully review the instructions provided with your specific antigen test kit. Different kits may have slightly different procedures.
  • Gather Supplies: Make sure you have all the necessary components: the swab, the test strip or cassette, the extraction buffer (liquid), and a tube or container for mixing.
  • Wash Your Hands: Wash your hands thoroughly with soap and water for at least 20 seconds. Alternatively, use hand sanitizer with at least 60% alcohol.
  • Prepare the Test Area: Find a clean, flat surface to perform the test.

2. Sample Collection:

  • Open the Swab Packaging: Carefully open the swab packaging, being careful not to touch the soft tip with your fingers.
  • Insert the Swab: Gently insert the swab into your left nostril. The correct depth is usually about 2cm to 4cm (approximately 1 inch). You should feel slight resistance. Do not force it in.
  • Swirl the Swab: Roll the swab 5 times against the inside wall of your nostril. Make sure you're making good contact with the nasal lining.
  • Repeat in the Other Nostril: Remove the swab from your left nostril and insert it into your right nostril. Repeat the swirling motion 5 times. This ensures you collect a sufficient sample from both nostrils.

3. Sample Processing:

  • Open the Extraction Buffer: Open the tube of extraction buffer solution provided in the kit.
  • Insert the Swab into the Tube: Insert the swab into the tube containing the extraction buffer.
  • Mix the Sample: Rotate the swab vigorously in the liquid at least 10 times. Press the swab head against the inside of the tube to release the sample into the buffer.
  • Remove the Swab: Remove the swab while squeezing the sides of the tube to extract as much liquid as possible. Discard the used swab appropriately.
  • Apply the Sample to the Test Strip: Follow the kit's instructions for applying the processed sample to the test strip or cassette. This usually involves squeezing a specified number of drops of the liquid onto the designated area of the test device.

4. Reading the Results:

  • Wait the Specified Time: Wait the amount of time indicated in the instructions (typically 10-15 minutes).
  • Interpret the Results: Read the results based on the lines or symbols that appear on the test strip or cassette. The kit instructions will provide detailed information on how to interpret the results (e.g., one line might indicate a negative result, while two lines indicate a positive result). Even a faint line should be considered a positive result.
  • Dispose of the Test: Dispose of the used test components properly, according to local regulations.

Important Considerations:

  • False Negatives: Antigen tests are less sensitive than PCR tests and may produce false negative results, especially early in an infection or in asymptomatic individuals.
  • Follow-Up Testing: If you have symptoms of COVID-19 and receive a negative antigen test result, consider getting a PCR test to confirm the result.
  • Consult a Healthcare Professional: If you have any questions or concerns about your results, consult a healthcare professional.

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