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What is the difference between urine culture and urine routine?

Published in Medical Testing 3 mins read

The key difference between a urine culture and a urine routine, also known as a urinalysis, is that a urine culture is specifically designed to identify bacterial infections, while a urine routine looks at a broader range of physical and chemical characteristics of the urine. Cultures are not part of routine urinalysis.

Here's a more detailed breakdown:

Urine Routine (Urinalysis)

A urine routine is a general test that analyzes a urine sample for several parameters. It is commonly used as an initial screening tool. These parameters include:

  • Physical Examination:
    • Color: Assessing for abnormalities like dark or cloudy urine.
    • Appearance: Checking for clarity or turbidity.
    • Specific gravity: Indicates the concentration of urine.
  • Chemical Examination:
    • pH: Measures the acidity or alkalinity.
    • Glucose: Detects the presence of sugar, which may indicate diabetes.
    • Protein: Checks for protein, which could suggest kidney problems.
    • Bilirubin: Looks for an indication of liver disease.
    • Blood: Detects blood, which might indicate infection or trauma.
    • Ketones: Detects products of fat breakdown, seen in uncontrolled diabetes.
    • Leukocyte esterase and Nitrites: Detects signs of infection.
  • Microscopic Examination:
    • Examines urine for the presence of cells such as red blood cells, white blood cells, and epithelial cells.
    • Looks for casts or crystals.

Urine Culture

A urine culture is a laboratory procedure specifically performed to identify and count bacteria present in a urine sample. It involves:

  • Inoculation: Placing a small amount of urine onto a sterile growth medium.
  • Incubation: Allowing the bacteria in the urine to grow in a controlled environment.
  • Identification: Identifying any bacterial species that grow and calculating the number of colony forming units (CFU).
  • Sensitivity testing (Antibiotic Susceptibility): Determining which antibiotics can effectively kill or inhibit the growth of the identified bacteria.

Key Differences Summarized

Feature Urine Routine (Urinalysis) Urine Culture
Primary Purpose General assessment of urine composition, initial screening Identify and quantify bacteria, diagnose urinary tract infections
Method Physical, chemical, and microscopic examination Growing bacteria in a laboratory setting
Detects Broad range of abnormalities, e.g., glucose, protein, blood, cells Specific bacteria, their quantity, and antibiotic sensitivities
Part of routine urinalysis? Yes No
When Used Routine checkups, screening for disease, monitoring kidney health Suspected urinary tract infection, recurrent infections

Practical Insights

  • Contamination: To obtain an uncontaminated urine sample for culture, a method to obtain an uncontaminated urine sample involves passing a catheter through the urethra into the bladder.
  • Follow-up: A urine routine might suggest an infection, prompting the need for a culture to pinpoint the causative bacteria and appropriate treatment.
  • Antibiotics: If a urine culture reveals a bacterial infection, the sensitivity test guides antibiotic selection.

In conclusion, while a urine routine offers a comprehensive overview of urine characteristics, a urine culture is targeted specifically to identify and manage bacterial infections.

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