Prepared slides, especially those with permanently mounted coverslips, require gentle cleaning to avoid damage. Here's the recommended method:
To clean prepared slides with permanently mounted coverslips, use a low-lint tissue and methylated spirit carefully.
Here's a breakdown:
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Materials Needed:
- Low-lint tissue (e.g., Kimwipes®)
- Methylated spirit (also known as denatured alcohol)
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Cleaning Procedure:
- Apply a Drop of Methylated Spirit: Place a single drop of methylated spirit onto the low-lint tissue. It's crucial to avoid using excess liquid.
- Gently Wipe the Slide: Carefully and gently wipe the marked areas of the slide.
- Avoid Excessive Pressure: Apply very light pressure to avoid scratching the slide or disturbing the coverslip.
- Check for Opacity: After cleaning, check the slide for any changes in opacity or issues with the coverslip adhesion. If the coverslip shows signs of lifting, stop cleaning immediately.
- Allow to Dry: Let the slide air dry completely before using it again. This ensures that any remaining methylated spirit evaporates.
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Important Considerations:
- Avoid Excess Liquid: Using too much methylated spirit can affect the opacity of the mounting medium and may cause the coverslip to detach.
- Gentle Approach: A gentle touch is essential to prevent damage to the slide or the coverslip.
- Alternative Cleaning Method (if necessary): For some slides, a very lightly damp cloth may remove dust before applying methylated spirit as described above. However, ensure the slide is dried immediately with a clean low-lint cloth.
