The wavelength of a laser scanning microscope is not a single value, but rather depends on the specific laser(s) used in the microscope.
The reference text indicates that laser scanning microscopes utilize various laser combinations, and specific wavelengths are selected using excitation filters. Examples provided include:
- 488 nm (blue) for fluorescein fluorescence.
- 568 nm (green) for rhodamine fluorescence.
- 647 nm (likely red).
Therefore, a laser scanning microscope isn't limited to one wavelength. It can operate at multiple wavelengths depending on the laser source and filter selection. The microscope utilizes different wavelengths to excite different fluorophores, allowing for the visualization of multiple structures or molecules within a sample.
In summary, there is no single wavelength for a laser scanning microscope; it uses a range of wavelengths, selected based on the application and the fluorophores being used.
