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Is Cas9 a protein?

Published in Molecular Biology 1 min read

Yes, Cas9 is indeed a protein.

Cas9, short for CRISPR-associated protein 9, is a vital component in the CRISPR-Cas9 gene editing system. According to the provided reference, Cas9 (formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein. This protein plays a crucial role in the immune defense of certain bacteria against DNA viruses and plasmids. It is also extensively used in genetic engineering applications.

Here's a breakdown of Cas9's characteristics:

  • Nature: Cas9 is a protein.
  • Size: It has a molecular weight of approximately 160 kDa (kilodaltons).
  • Function: It acts as a molecular "scissor" to cut DNA at specific locations guided by an RNA molecule.
  • Origin: It's naturally found in bacteria as part of their immune system.
  • Application: It's widely used in gene editing to modify DNA sequences in various organisms.

Therefore, based on the information provided, the answer is definitively yes.

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