Gene clean, as defined by the GENECLEAN® kit, refers to a simplified process for purifying DNA fragments. It involves three main steps: bind, wash, and elute. This method eliminates the need for ethanol precipitation, resulting in purified DNA ready for a variety of applications. The kit is designed to purify DNA fragments ranging from 200 base pairs (bp) to 20 kilobase pairs (kb).
How GENECLEAN® Works
The GENECLEAN® kit provides a straightforward approach to DNA purification. Here's a breakdown of each step:
- Bind: DNA fragments are bound to a specialized matrix within the kit's columns.
- Wash: Impurities and other contaminants are washed away, leaving only the bound DNA.
- Elute: The purified DNA is released or eluted from the matrix, ready for use in downstream applications.
Benefits of Using GENECLEAN®
- Simplicity: The three-step process is easy to follow and reduces the complexity of DNA purification.
- Speed: The process is quick and efficient, saving time compared to traditional methods.
- Efficiency: The method avoids the use of ethanol precipitation, streamlining the protocol.
- Versatility: The purified DNA is suitable for a broad range of downstream applications such as:
- PCR amplification
- DNA sequencing
- Restriction digestion
- Cloning
DNA Fragment Size Range
The GENECLEAN® kit is effective for purifying DNA fragments within a specific size range:
| Feature | Details |
|---|---|
| Minimum Size | 200 base pairs (bp) |
| Maximum Size | 20 kilobase pairs (kb) |
This makes it suitable for a wide range of molecular biology applications.
