The primary difference between Cre and FLP lies in their optimal reaction temperatures: Cre recombinase functions best at 37°C, while FLP recombinase is most effective at 30°C.
While both Cre-loxP and Flp-FRT systems serve a similar purpose - inducing gene recombination - their differing optimal temperatures can be a crucial factor when choosing which system to use for specific experimental conditions. This difference can affect the efficiency and specificity of the recombination process.
Here's a breakdown:
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Cre-loxP System: Cre recombinase recognizes specific DNA sequences called loxP sites. When Cre is present, it catalyzes recombination between loxP sites, leading to excision, inversion, or translocation of the DNA segment flanked by the loxP sites. Optimal temperature: 37°C.
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Flp-FRT System: FLP recombinase recognizes specific DNA sequences called FRT sites. Similar to Cre, FLP mediates recombination between FRT sites. Optimal temperature: 30°C.
In summary, while both are recombinases used for site-specific genetic manipulation, the difference in optimal temperature is a key consideration when selecting the appropriate system.
