The best way to store RNA to prevent degradation is by freezing it at very low temperatures.
Why is RNA Storage Important?
RNA is a delicate molecule that can degrade quickly due to the action of ribonucleases (RNases), enzymes that break down RNA. Proper storage is crucial to maintain the integrity of RNA samples for research, diagnostics, and other applications.
Optimal Storage Conditions for RNA
Based on the provided reference, the optimal way to store RNA is to keep it frozen at −20 °C, −80 °C, or under liquid nitrogen. Here is a breakdown of the best practices:
Freezing Temperatures
| Temperature | Description |
|---|---|
| -20°C | Suitable for short-term storage. |
| -80°C | Preferred for long-term storage, maintains RNA integrity. |
| Liquid Nitrogen | Provides the most stable storage by halting all enzyme activity, ideal for extremely long-term storage. |
Considerations
- Even at low temperatures such as -20 °C, some RNase activity can persist. For long term storage -80 °C or liquid nitrogen should be used.
Practical Storage Solutions
Here are some practical insights for storing RNA:
- Use RNase-free consumables: Always use sterile, RNase-free tubes, tips, and reagents.
- Aliquot RNA: Divide large RNA samples into smaller aliquots to avoid repeated freeze-thaw cycles, which can damage RNA.
- Rapid Freezing: Snap-freeze RNA samples quickly in liquid nitrogen or using a dry ice/ethanol bath before moving them to the final storage temperature to ensure stability.
- Proper Labeling: Label all tubes clearly with date, content, and storage information.
- Storage containers: Store samples in a freezer at the designated temperature or a liquid nitrogen tank, making sure they are sealed properly to prevent moisture build-up.
