Saliva samples are transferred using various methods depending on the collection technique.
Saliva Collection Methods and Transfer
The method of saliva sample transfer often depends on how the saliva is initially collected. Here are some common methods and how they facilitate transfer:
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Passive Drool Method: In this method, individuals allow saliva to pool in their mouth and then drool it directly into a collection container, such as a sterile tube. This is a direct transfer from the mouth to the container. This method is preferred by most researchers to maintain consistency in the sample collected, as noted in the provided reference: "Most researchers prefer to use unstimulated, whole saliva collected by the passive drool technique in order to maintain consistency in the type of sample collected."
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Absorbent Device Method: This method involves using swabs or other absorbent materials placed in the mouth to collect saliva. The saliva-saturated swab is then transferred to a collection tube or container. Sometimes, the saliva is extracted from the swab before transfer. The reference mentions: "Absorbent Device Method: Swabs can be placed in the mouth to collect saliva for increased ease of use."
Key Considerations for Saliva Transfer
Regardless of the specific method, certain considerations are crucial when transferring saliva samples:
- Sterility: Maintaining a sterile environment is essential to prevent contamination of the sample. Sterile containers and handling techniques are necessary.
- Temperature Control: Depending on the analysis to be performed, saliva samples may need to be kept at a specific temperature (e.g., refrigerated or frozen) during and after transfer to maintain sample integrity.
- Labeling: Proper labeling of the collection container with patient information, date, and time of collection is vital to avoid mix-ups.
- Speed: In some cases, prompt transfer and processing are needed to preserve the integrity of specific biomarkers or components in the saliva.
