Yes, Jane Gloriana Villanueva does lose her virginity.
After being a virgin for 25 years, and after getting married and having a baby, Jane finally loses her virginity. This pivotal moment occurs during the course of the television series Jane the Virgin. The show dedicates a significant portion of its narrative to Jane's journey as a young, religious woman navigating the complexities of relationships, motherhood, and her eventual loss of virginity.
