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Is the C line or T line positive?

Published in Test Result Interpretation 2 mins read

The question of whether the C line or T line is positive depends on the context of a test, specifically in scenarios like lateral flow assays (e.g., rapid antigen tests). In these tests, a positive result is indicated when both the Control (C) line and the Test (T) line are visible.

Here's a breakdown of what each line signifies and what it means for the test outcome:

Understanding the Lines

Line Meaning
Control (C) Line This line is a procedural control, indicating that the test has been performed correctly and the reagents are working as intended. It must be visible for the test result to be valid.
Test (T) Line This line appears only if the target substance (e.g., antigen) is present in the sample at a detectable level. A visible T line indicates a positive result.

Interpreting the Results

According to the provided reference:

  • If the Control (C) line and the Test (T) line are visible, the test is positive. Any faint visible test (T) line, along with the control line (C), is considered a positive result.
  • A positive result means that the target substance being tested for is present in the sample.
  • A positive result at any time during testing means repeat testing is unnecessary.

In summary, if both the C line and the T line are visible, then the test is positive. It is not correct to label just one line as “positive,” rather the combination of both lines determines positivity.

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