Tissue samples are collected using a sharp and sterile instrument, typically a scalpel or razor blade. Avoid using scissors, as they can damage the tissue.
Here's a breakdown of the process:
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Preparation:
- Ensure you have a sterile scalpel or razor blade.
- Prepare appropriate fixative (e.g., formalin) in a container.
- Gather necessary personal protective equipment (PPE), such as gloves.
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Collection:
- Using the sterile scalpel or razor blade, carefully excise the tissue sample.
- Avoid crushing or tearing the tissue during the process.
- Aim for a sample size of no more than 1 cm3 (approximately 0.6 in3) to ensure adequate fixative penetration. Larger samples may not be properly preserved in the center.
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Fixation:
- Immediately place the collected tissue sample into the prepared fixative.
- Ensure the tissue is fully submerged in the fixative.
- Use an adequate amount of fixative (typically 10-20 times the volume of the tissue).
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Documentation:
- Properly label the sample container with relevant information (patient ID, date, tissue type, etc.).
- Maintain a detailed record of the sampling process.
Why Scalpels/Razor Blades are Preferred over Scissors:
Scissors tend to crush the tissue as they cut, distorting the cellular architecture. This distortion can interfere with accurate microscopic examination. Scalpels and razor blades provide a cleaner, less traumatic cut.
In summary, tissue sample collection requires the use of a sharp, sterile instrument like a scalpel or razor blade, careful handling to avoid tissue damage, and prompt fixation to preserve the tissue's structure.
