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What is dye factor?

Published in Vitamin C Analysis 1 min read

The dye factor, F, represents the mass, measured in milligrams (mg), of vitamin C that is equivalent to 1 cubic centimeter (cm3) of a DCPIP (2,6-dichlorophenolindophenol) solution.

In simpler terms, the dye factor tells you how much vitamin C a specific volume of DCPIP solution can react with during a titration. It's a crucial value for determining the concentration of vitamin C in a sample.

The process typically involves titrating a known concentration of vitamin C with the DCPIP solution until the DCPIP loses its color. The volume of DCPIP used is then related to the amount of vitamin C present, using the dye factor.

Therefore, if you have a dye factor of, for example, 0.5 mg/cm3, it means that 1 cm3 of your DCPIP solution is equivalent to 0.5 mg of Vitamin C.

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