Based on the provided reference, the question "How to make a yeast cell?" is best understood in the context of preparing competent yeast cells, meaning cells that are ready to take up foreign DNA in a process called transformation. The provided snippets describe steps in preparing yeast cells for this purpose. Therefore, the following answer describes how to prepare competent yeast cells, not how yeast cells are made in general through reproduction.
To prepare competent yeast cells for transformation, you would follow these steps:
Steps for Preparing Competent Yeast Cells
Here's a breakdown of the process to prepare yeast cells so they're ready to take up DNA:
-
Culturing: Begin by culturing your desired yeast strain overnight. This allows the yeast to grow to a suitable density for further processing (Reference: "Culture the yeast strain(s) you want to transform overnight...").
-
Harvesting: Once the yeast reaches the appropriate cell density (usually 1 to 2 x 107 cells/mL), harvest them by centrifugation (Reference: "Harvest cells by centrifugation once they reach the right cell density (usually 1 to 2 x 107 cells/mL)..."). This concentrates the cells into a pellet.
-
Washing: Wash the cells to remove any residual growth medium. This is done by:
- Resuspending the cell pellet in sterile water.
- Centrifuging again to pellet the cells and discarding the water (Reference: "Wash cells by resuspending them in sterile water and centrifuge to discard water with any traces of medium."). This washing step ensures that no traces of the original medium interfere with the transformation process.
